Open resource · 2024-2025 data

HPLC Purity of Research Peptides

A side-by-side analysis of 12 peptides: EXOMA Peptides's per-lot verified purity vs. the average reported by industry suppliers. Methodology, raw data and FAQs.

◈ 12 compounds◎ COA per lot↗ Updated Q1 2025

Purity table by compound

Purity percentage measured by reversed-phase HPLC (C18). Lab values are the average of the last 3 lots analyzed.

Compound	CAS	EXOMA Peptides (%)	Industry (%)	Comparison
BPC-157	137525-51-0	99.2%	92%	+7.2%
TB-500	77591-33-4	99.1%	90%	+9.1%
Tirzepatida	2023788-19-2	99.3%	91%	+8.3%
Retatrutida	2381089-83-2	99.5%	89%	+10.5%
GHK-Cu	49557-75-7	99%	88%	+11.0%
Epithalon	307297-39-8	99.1%	87%	+12.1%
Ipamorelin	170851-70-4	99.3%	91%	+8.3%
CJC-1295	863288-34-0	99.2%	90%	+9.2%
PT-141	32780-32-8	99%	89%	+10.0%
Selank	129954-34-3	99.1%	86%	+13.1%
DSIP	62568-57-4	99.2%	88%	+11.2%

BPC-157

137525-51-0

+7.2%

EXOMA Peptides 99.2%

Industry 92%

TB-500

77591-33-4

+9.1%

EXOMA Peptides 99.1%

Industry 90%

Tirzepatida

2023788-19-2

+8.3%

EXOMA Peptides 99.3%

Industry 91%

Retatrutida

2381089-83-2

+10.5%

EXOMA Peptides 99.5%

Industry 89%

GHK-Cu

49557-75-7

+11.0%

EXOMA Peptides 99%

Industry 88%

Epithalon

307297-39-8

+12.1%

EXOMA Peptides 99.1%

Industry 87%

Ipamorelin

170851-70-4

+8.3%

EXOMA Peptides 99.3%

Industry 91%

CJC-1295

863288-34-0

+9.2%

EXOMA Peptides 99.2%

Industry 90%

PT-141

32780-32-8

+10.0%

EXOMA Peptides 99%

Industry 89%

Selank

129954-34-3

+13.1%

EXOMA Peptides 99.1%

Industry 86%

DSIP

62568-57-4

+11.2%

EXOMA Peptides 99.2%

Industry 88%

* «Industry» values represent the weighted average purity reported by 15 international suppliers based on public COA data (2023-2025). EXOMA Peptides values are the average of the last 3 lots analyzed by an accredited external lab.

HPLC Methodology

How a peptide's purity is analyzed step by step. Protocol based on USP guidelines and adapted for synthetic peptides.

Step 1

Sample preparation

Dissolution in mobile phase with 0.22μm filtration to remove particles that interfere with the chromatographic reading.

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Step 2

Chromatographic separation

Reversed-phase C18 column (250×4.6mm, 5μm). Acetonitrile/water gradient with 0.1% TFA. Flow 1.0 mL/min.

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Step 3

UV detection at 220nm

Wavelength optimized for peptide bonds. 20μL injection with a diode-array detector (DAD).

Step 4

Purity calculation

Area-normalization method: % purity = (main peak area / total area) × 100. All impurities >0.1% are reported.

High Purity

Minimum purity guaranteed across all our peptides

+7.2%

Average advantage vs. the industry average

100%

Lots with an individually verifiable COA

FAQs about HPLC purity

What does 99%+ purity mean in peptides?+

It indicates the peptide has been verified by high-performance liquid chromatography (HPLC), confirming that more than 99% of the content is the intended compound, with no significant contaminants.

Why does purity matter in research peptides?+

Impurities can alter experimental results and reproducibility. High, verifiable purity ensures the data reflect the studied compound and not synthesis artifacts.

What is a per-lot COA?+

The Certificate of Analysis (COA) details each lot's quality results: purity by HPLC, identity by mass spectrometry, endotoxin content and sterility.

How is the purity percentage calculated?+

By area normalization on the HPLC chromatogram: purity is the main peak area divided by the total area of all peaks, multiplied by 100.

Cite and share this data

This resource is free to use under a CC BY-SA 4.0 ↗. You may cite it, embed the table or link directly from your blog, forum or scientific publication.

Suggested citation:

EXOMA Peptides. «HPLC Purity Comparison in Research Peptides, 2024-2025.» Available at: https://exoma-peptides.com/pureza-hplc.

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