Step-by-step guide to safely prepare lyophilized peptides with bacteriostatic water
Important: This guide is for informational and research purposes only. Always consult a professional before handling any substance.
Calculate the exact amount of bacteriostatic water for your peptide and desired dose
Gather the vial of lyophilized peptide, bacteriostatic water, insulin syringes and alcohol wipes. Make sure everything is clean and sterile.
Clean the rubber stopper of the vial with an alcohol wipe and let it dry for 30 seconds before inserting the needle.
With the syringe, draw the desired amount of bacteriostatic water. For most peptides, 1-2 mL is enough. Check the calculator for specific doses.
Insert the needle into the peptide vial and inject the water SLOWLY down the side of the vial. Let the water trickle down the wall. Never inject it directly onto the powder.
Let the vial sit for 5-10 minutes. The peptide will dissolve gradually. Do NOT shake the vial; swirl it gently if needed.
Once reconstituted, store the vial refrigerated (2-8°C). Most reconstituted peptides last 3-4 weeks refrigerated.
Denatures the peptide's proteins, reducing its effectiveness
Can damage the molecular structure of the lyophilized compound
Introduces contaminants that can degrade the peptide
The reconstituted peptide degrades quickly outside of 2-8°C
The amount depends on the peptide and the desired concentration. For most 5 mg peptides, 1-2 mL of bacteriostatic water is used. Use our reconstitution calculator to work out the exact amount for your dosing.
A peptide correctly reconstituted with bacteriostatic water lasts 3-4 weeks refrigerated at 2-8°C. Without refrigeration, it degrades within hours. Never freeze a peptide once it has been reconstituted.
It is not recommended for multi-dose use. Bacteriostatic water contains 0.9% benzyl alcohol, which prevents bacterial growth. Sterile water is only suitable for immediate, single-dose use.
Signs of degradation include: a cloudy solution or visible particles, a color change, an unusual odor, or loss of effectiveness. If you notice any of these signs, discard the vial.
Find bacteriostatic water and every research compound in our full catalog.
